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KMID : 0624620170500010031
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BMB Reports
2017 Volume.50 No. 1 p.31 ~ p.36
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Caffeine-induced food-avoidance behavior is mediated by neuroendocrine signals in Caenorhabditis elegans
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Min Hye-Min
Youn Esther
Kawasaki Ichiro
Shim Yhong-Hee
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Abstract
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High-dose caffeine uptake is a developmental stressor and causes food-avoidance behavior (aversion phenotype) in C. elegans, but its mode of action is largely unknown. In this study, we investigated the molecular basis of the caffeine-induced aversion behavior in C. elegans. We found that aversion phenotype induced by 30 mM caffeine was mediated by JNK/MAPK pathway, serotonergic and dopaminergic neuroendocrine signals. In this process, the dopaminergic signaling appears to be the major pathway because the reduced aversion behavior in cat-2 mutants and mutants of JNK/MAPK pathway genes was significantly recovered by pretreatment with dopamine. RNAi depletion of hsp-16.2, a cytosolic chaperone, and cyp-35A family reduced the aversion phenotype, which was further reduced in cat-2 mutants, suggesting that dopaminergic signal is indeed dominantly required for the caffeine-induced food aversion. Our findings suggest that aversion behavior is a defense mechanism for worms to survive under the high-dose caffeine conditions.
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KEYWORD
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Caenorhabditis elegans, Caffeine, Food-avoidance behavior, JNK/MAPK pathway, Neuroendocrine signals
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